Abstract

REMIGIO MONTERO, Antonia de la Caridad et al. Genotoxic assessment of hydroalcoholic extract of Tamarindus indica L using micronuclei assay in mouse bone marrow. Rev Cubana Plant Med [online]. 2007, vol.12, n.2, pp. 0-0. ISSN 1028-4796.

Micronuclei assay in mouse bone marrow, test included in genotoxicity standard battery, was used to assess clastogenic and aneugenic potential of a hydroalcoholic extract of Tamarindus indica L. We used NMRI mice of both sexes, with administration by intragastric route at a rate of 10 mL/kg, in the space of 24 hours, sacrified 24 hours after last application. Genotoxicity rate, polychromatic micronucleated erythrocyte percentage in a total of 1 000 polychromatic erythrocytes per animal. Genotoxicity rates of treated groups using 500, 1000, and 2000 mg/kg extract/body weight were statistically significant as for group treated with control substance (H2O sterile). Extract of Tamarindus indica L was of clastogenic type, since give raise to a significant increase in micronuclei frequency of bone marrow polychromatic erythrocytes, and in ratios linear trend test showed a dosis-response relationship.

Keywords : Genotoxicity; bone marow; micronuclei; Tamarindus indica L.