Abstract

PUERTAS-MEJIA, Miguel A; RIOS-YEPES, Yersica  and  ROJANO, Benjamín Alberto. Determination of anthocyanins by microwave assisted extraction in beans (Phaseolus vulgaris L.) commonly consumed in Antioquia, Colombia. Rev Cubana Plant Med [online]. 2013, vol.18, n.2, pp. 288-297. ISSN 1028-4796.

Introduction: apart from being an important nutritional source in Colombia, beans also provide a large amount of substances with potential beneficial effects on health, such as flavonoids and anthocyanins, among others, which stand out for their medicinal properties and potential positive action against some chronic diseases. Objectives: compare the content of anthocyanins in the pericarp of the fruit of different varieties of beans with reference to the extraction method. Methods: after dehydration and maceration, the bean fruit coat was subjected to solid-liquid and microwave assisted extraction with acidulated methanol. Anthocyanin content was determined by the pH differential method, and the cation radical α-α-diphenyl-ß-picrylhydrazyl (DPPH) method was used for determination of in vitro antioxidant potential. Results: microwave assisted extraction (MAE) proved to be more efficient than the conventional technique, with a considerable reduction in the amount of solvent, sample and extraction time. Anthocyanin content in the extracts obtained was between 8.33 and 25.5 mg cyanidin-3-glucoside/L. However, their antioxidant protection effect was similar and comparable to that of reference substances. Conclusions: all extracts showed good protective capacity against free radicals. Microwave assisted extraction may be used as an alternative method for fast, efficient and effective evaluation of the content of bioactive substances in various matrices.

Keywords : natural antioxidants; anthocyanins; microwave assisted extraction (MAE); bean; Phaseolus vulgaris L.; pericarp.

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