Abstract

PUIG PENA, Yamila; CARRERA VARA, José; LEYVA CASTILLO, Virginia  and  HERNANDEZ CASTRO, Idalmis. Establishment of a technique to determination of entero-parasites in vegetables by immunofluorescence. Rev Cubana Hig Epidemiol [online]. 2011, vol.49, n.1, pp. 24-32. ISSN 1561-3003.

There are biological risks associated with vegetables including the human pathogen parasites, thus it was necessary to establish a technique to assessment of entero-parasites in these foods. This procedure was applied according to the method described in the FDA Bacteriological Analysis Manual / CFSAN of 2001 where ten vegetable samples were investigated from five culture areas from the Ciudad de La Habana province from January to March, 2009. Authors used a direct examination with concentrated Lugol's solution and the immunofluorescence technique for Giardia and Cryptosporidium by monoclonal antibodies kit (MERIFLUOR C/G). For control of parasites extraction process we took a sample of lettuce adding a suspension based in Giardia cysts. By direct examination we achieved the 14,4% of recovery and by immunofluorescence the 57,8%. Results obtained for immunofluorescence control were the expected ones. In two assay samples there were Giardia cysts by immunofluoresence but not Cryptosporidium cysts.

Keywords : Parasite; vegetables; immunofluorescence; Giardia; Cryptosporidium.

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