Abstract

BARO ROBAINA, Yamilé; FONTANA CAPALBO, Deise M  and  DOS SANTOS NASCIMENTO, Rosely. Caracterization of Bacillus thuringiensis Berliner Strains and Biological Activity on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) and Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). Fitosanidad [online]. 2009, vol.13, n.3, pp. 187-192. ISSN 1818-1686.

This study describes the characterization of nine Cuban Bacillus thuringiensis strains based on crystal morphology, SDS polyacrylamide electrophoresis (PAGE) and insecticidal activity against Spodoptera frugiperda and Anticarsia gemmatalis.Ultrastructural analysis of parasporal bodies of the nine strains showed the typical bipyramidal crystal and cubic inclusion partially embedded in the middle of the bipyramidal crystal. The PAGE analysis showed two bands of 130 kDa and 70 kDa belongs to Cry 1 and Cry 2 protein present to HD1 standard strains B. thuringiensis var. kurstaki.The strains LBT 4 and LBT 7 analyzed in this report showed potential as biological insecticide against S. frugiperda and LBT 4, LBT 7, LBT13 and LBT47 strains showed 100% of mortality to Anticarsia gemmatalis.

Keywords : Bacillus thuringiensis; d-endotoxins; Spodoptera frugiperda; Anticarsia gemmatalis; biological activity.

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