Abstract

MOREJON-ALONSO, Loreley; WILSON-SAVON, Liem; FERNANDEZ-MASSO, Julio R.  and  DIAZ-AGUILA, Carlos. Magnetic iron oxide nanoparticles for plasmid DNA isolation. Rev Cub Quim [online]. 2020, vol.32, n.2, pp. 195-217.  Epub Sep 21, 2020. ISSN 2224-5421.

The aim of this work was the synthesis and characterization of iron oxide magnetic nanoparticles coated with silica (Fe3O4@SiO2) intended for plasmid DNA isolation. Synthesis was carried out through co-precipitation method and coating was performed used the Stöber method. Materials were characterized by X-ray fluorescence, X-ray diffraction and Infrared spectroscopy. DNA purification was performed by comparing the ethanolic precipitation method, the QIAprep® commercial DNA purification kit and the synthesized Fe3O4@SiO2. Results showed that is possible to obtain Fe3O4@SiO2 (ranging size from 9 to 12 nm) through the proposed methodology. The amount of Si adsorbed onto the surface of magnetic particles and the crystal size of the Fe3O4@SiO2 were pH and washing-conditions dependent. The isolation assay showed that it is possible to recovery efficiently plasmid DNA using the Fe3O4@SiO2 obtain in normal conditions as solid support.

Keywords : nanoparticles; magnetite; silica; Stöber; DNA extraction.

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