Abstract

ESCOBEDO-NICOT, Miriela Milagros et al. Evaluation of the anti-angiogenic activity of methyl jasmonate in chorioallantoic membrane of chicken embryos by means of digital image analysis. Rev Cub Quim [online]. 2020, vol.32, n.3, pp. 527-542.  Epub Dec 03, 2020. ISSN 2224-5421.

A study is presented to measure the effect of Methyl Jasmonate (MJ) on angiogenesis in chorioallantoic membrane (CAM) samples from chicken embryos, using an imaging analysis software. The characteristics considered are: terminal points, bifurcation points, area density, vascular length and fractal dimension, among others. In addition, the classification of blood vessels using Strahler and Tokunaga taxonomies is included, which allows classifying segments of hierarchical structures. For the evaluation of the results, control samples and others treated with MJ in water and liposomes are used in three concentrations 7.5, 15 and 30 µM. The anti-angiogenic tendency was corroborated in the environment of liposomes considering the decrease of the quantitative values of all the evaluated characteristics with regard to the control samples. The results obtained agree with data from literature regarding the best bioavailability associated to the lipid vehicle.

Keywords : quantification; angiogenesis; methyl jasmonate; CAM.

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