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Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
Abstract
TORRES, DINORAH; FERNANDEZ, MARIBEL; BRITO, TANIA and FINLAY, CARLOS. Ensayo inmunoenzimático en fase sólida para la detección de antígenos de Giardia lamblia . Rev Cubana Med Trop [online]. 1997, vol.49, n.1, pp. 52-58. ISSN 1561-3054.
An ELISA sandwich for the detection of Giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from G. lamblia. The sensitivity of the technique was 94,8 % and the specificity 98,3 %. The method detects an antigen concentration of 31 ng. The procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.
Keywords : GIARDIA LAMBLIA [immunology]; ANTIGENS, PROTOZOAN; ENZYME-LINKED IMMUNOSORBENT ASSAY [methods]; FAECES [parasitology].