Abstract

MORIER, Luis et al. Comportamiento biológico de 3 cepas del virus dengue-2 en 2 líneas celulares de mosquitos. Rev Cubana Med Trop [online]. 2000, vol.52, n.3, pp. 215-219. ISSN 1561-3054.

Strains A-15 (isolated in Cuba, 1981), Jamaica (isolated in Jamaica, 1981) and Nueva Guinea "C" (standard) from dengue-2 virus were compared according to the time of appearance of the cytopatic effect (CPE), to the time of appearance of specific fluorescence and to the kynetics of viral multiplication on being innoculated in the cell lines AP-61 (Aedes pseudoscutellaris) and C6/36 HT (Aedes albopictus). The results showed that the CPE of highest intensity and earliest appearance was for A-15, followed by Jamaica and Nueva Guinea "C" (NGC). AP-61 seems to favor the CPE of Jamaica with respect to that of the same strain in C6/36 HT. The fluorescence was earlier for Jamaica and A-15 and more intensive for the latter, whereas NGC manifested late. This behaviour was similar in the 2 cellular systems. The greatest titres during the kynetics of viral multiplication were obtained from A-15 in both lines, although in AP-61 they tend to be equal from the 4th day on. The strain A-15 showed a particular behaviour of these biological properties on comparing them with the other strains under study, which may be related to changes found in its neuclotide sequence.

Keywords : DENGUE; AEDES; CYTOPATHOGENIC EFFECT; VIRAL [immunology]; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT [methods].

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