Abstract

MARTIN ALFONSO, Dayamí et al. Design and evaluation of DAVIH VIH-2 . Rev Cubana Med Trop [online]. 2007, vol.59, n.3, pp. 0-0. ISSN 1561-3054.

The results of the design and evaluation of DAVIH VIH-2 diagnosing system, an indirect Elisa for screening of HIV-2 antibodies, which uses a HIV-2 glycoprotein gp36 synthetic peptide in its solid phase, were exposed. In the system evaluation using WHO reference panels, 100 % sensitivity, 99,81 % specificity, 99,81% efficacy and very good concordance level (kappa = 0.978) were attained. Serum samples of 959 individuals with undetermined or negative results to the HIV-1 antibodies confirmation (DAVIH blot) were evaluated by the DAVIH VIH-2 system. Twenty four samples were reactive, six of which had confirmed HIV-2 antibodies. These results allowed recommending the introduction of this diagnostic kit in the HIV infection diagnosing algorithm in Cuba.

Keywords : HIV-2 antibodies; AIDS serodiagnosis; validation studies; ELISA /methods.

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