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Revista Cubana de Hematología, Inmunología y Hemoterapia
On-line version ISSN 1561-2996
Abstract
MORALES, Maydelin Miguel et al. Alkaline phosphatase isoenzyme electrophoresis: diagnostic utility in patients with sickle cell disease. Rev Cubana Hematol Inmunol Hemoter [online]. 2022, vol.38, n.4 Epub Dec 01, 2022. ISSN 1561-2996.
Introduction:
Human alkaline phosphatases (ALP) are enzymatic indicators of organ damage. Complications of sickle cell disease include injury to organs due to reperfusion injury, proliferative vascular disease, and hemolytic anemia.
Objective:
To demonstrate the usefulness of determing ALP activity and the electrophoretic mobility of the serum ALP isoenzymes in patients with sickle cell disease at base line and during the crises.
Methods:
A descriptive, longitudinal, prospective study was carried out in 85 adult individuals, 25 healthy controls and 60 patients, between January and December/2018. Studied variables: genotypes (SS/SβO, SC/Sβ+), age, sex, ALP activity and isoenzymes by agarose gel electrophoresis with and without lectin: hepatic 1 (H1) and (H2); placenta 1 (P1), bone, intestinal 1, 2, 3 (I1.2.3) in basal state and crisis.
Results:
ALP activity was significantly higher in patients than in controls. Significant differences were found in the activity of the ALP and fraction H1+P1/1 of patients in basal state in relation to the control group. The activity of the enzyme and the isoenzymes showed no significant differences between genotypes. The same behavior was observed in the activity of enzymes and isoenzymes during painful and liver vasooclusive crises. Significant differences were found in the activity of the liver fraction H1+P1/1 between the groups of 20-29 and 40-49 years.
Conclusions:
The determination of the ALP in patients with sickle cell disease is useful and allows to establish a personalized isoenzimatic profile, with prognostic importance as a biological alarm marker.
Keywords : sickle cell disease; alkaline phosphatase; isoenzymes; electrophoresis.