Abstract

GARCIA CASTILLO, Elsa et al. Correlación del ADN y del antígeno de superficie del virus de hepatitis B en tejidos de animales transgénicos. Rev Cubana Invest Bioméd [online]. 2000, vol.19, n.1, pp. 8-10. ISSN 1561-3011.

A study was made on the presence of DNA coding for Hepatitis B surface antigen (HbsAg) in hybrid transgenic /Balb-c mice where the phenotype had not been expressed before. The extracted organs were evaluated by immunohistochemistry, by quantitative ELISA and polymerase chain reaction (PCR) for detecting viral DNA which was found in the studied tissues except for the intestines in which none of the species was evident. The immunohistochemical technique did not manage to detect the expression of viral protein in the intestines, muscles and seminal vesicle, only a very low expression of such protein in the brain. The immunoenzymatic technique did not detect HbsAG in muscles, intestines, brain or stomach. However, when evaluationg the sera of all the animals by immunoenzymatic technique and PCR, 10% pf tje sera were positive to viral DNA

Keywords : HEPATITIS B SURFACE ANTIGENS; POLYMERASE CHAIN REACTION; DNA, VIRAL; MICE TRANSGENIC; MICE, INBRED BALB C; TISSUES.

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