Abstract

GONZALEZ RAMIREZ, Victoria E et al. Método de inmunocomplejos específicos para detectar ADN del virus de hepatitis B en pacientes con antigenemias bajas. Rev Cubana Invest Bioméd [online]. 2000, vol.19, n.1, pp. 11-13. ISSN 1561-3011.

We evaluated the influence of the concentrations of particles associated with Hepatitis B virus from the immunologic viewpoint through the formation of specific antibody-antigen complexes, and also we determined DNA before and after the formation of the complexes. We used  10 serological samples of chronic carrier patients with low antigenemia(less than 1µg/mL) which were subjected to PCR for detecting viral DNA. The antibody-antigen complex technique was used in each sample through standard antibodies for the complex to be formed in vitro,  then purification with phenol  chrololform to be PCR-processed. The amplification of samples with the antibody-antigen complexes showed that this technique was able to concentrate antigens, so it could be detected when it was impossible to determine antigen concentrations in an agar electrophoresis

Keywords : ANTIGEN- ANTIBODY COMPLEX; HEPATITIS B VIRUS [immunology]; DNA VIRAL/IMMUNOILOGY; HEPATITIS B SURFACE ANTIGENS; POLYMERASE CHAIN REACTION [methods].

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