Abstract

LEBEQUE PEREZ, Yamila et al. Utilización de formaldehído y tratamiento térmico en la inactivación de cultivos de Pseudomonas aeruginosa. Rev Cubana Invest Bioméd [online]. 2003, vol.22, n.4, pp. 232-236. ISSN 1561-3011.

The inactivation of culture in a nutrient broth of Pseudomonas aeruginosa (serotype 011) was evaluated by using formaldehyde at concentrations of 0,1, 0,3, 0,6, 1 and 3% and inactivation by heat treatment using a bain-marie at 100°C. The effectiveness of both methods was determined by visually evaluating the growth of the inactivated culture on plates with tryptone soy agar. Regarding necessary formaldehyde concentration for obtaining the total inactivation of cultures in 24hours, the best results were achieved in variants that applied formaldehyde at 1 and 3%. The study showed that it is convenient to use the heat treatment for isolation and purification of heat-stable cellular components because it is very effective and allows optimizing the inactivation process time.

Keywords : PSEUDOMONAS AERUGINOSA [isolation & purification]; FORMALDEHYDE; DRUG RESISTANCE, MICROBIAL; THERMIC TREATMENT.

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