Abstract

MORENO, Damaris et al. Gen conversion and reversion events in Saccharomyces cerevisiae: A model for the study of gamma radiation damage. Rev Cubana Invest Bioméd [online]. 2004, vol.23, n.2, pp. 80-86. ISSN 1561-3011.

Radiosensitivity and kinetics of induction of gene conversion and reversion events in Sacharomyces cerevisiae strain D7 to gamma radiation at dose ranges from 100 to 800 Gy and 50 to 300 Gy respectively were studied. A source of ⁶⁰Co PX-g-30 at a dose rate of 49,43 GY/min was utilized. The cell survival curve showed DL₅₀ of 150 Gy. Cell death kinetics was linear and adjusted over 98 %. The induction of gene conversion events was significant in relation to control from 50 Gy on. However, gene reversion was significant only at 200 Gy. Generally speaking, gene conversion event frequencies were higher than those of reversion, which indicates that gamma radiation preferably induces recombinogenic events. Both the conversion and reversion events showed exponential dependence on gamma radiation dose. The relative benefits of this test for mutagenesis and anti-mutagenesis studies were debated in this paper

Keywords : ADN damage, gamma radiation; gene conversion and reversion; Sacharomyces cerevisiae.

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