Abstract

ALMEIDA, Eliseo et al. Effect of gamma radiation on the survival  and the induction of the SOS response in Eschericia coli  cells deficient in the repair by  excision of neuclotides and  recombination. Rev Cubana Invest Bioméd [online]. 2004, vol.23, n.4, pp. 242-248. ISSN 1561-3011.

The primary damages produced by the gamma radiation in the DNA of Escherichia coli strains carriers of the sulA::lacZ genic fusion and of mutations  in different DNA repair genes were studied. Its radiosensititvity was also determined. The strains with mutations in the recN and uvrA genes were more sensitive than the wild type. This agrees with previous studies where it was proved that the rec and uvr genes take part in the repair of the DNA damage produced by the gamma rays. In the mutant strains, there were found significant differences at the level of expression of the sulA gene in relation to the wild type. The usefulness of the strains studied as genotoxicity biosensors and in radioprotection studies was discussed.

Keywords : Gamma radiation; DNA repair; SOS response; Escherichia coli.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )