Abstract

HERNANDEZ BETANCOURT, Oscar et al. In vitro toxicity assessment caused by Rophalurus junceus scorpion poison though a cellular assay. Rev Cubana Invest Bioméd [online]. 2009, vol.28, n.1, pp. 0-0. ISSN 1561-3011.

Poison of blue scorpion (Rophalurus junceus) is marketed as Escozul. This natural product is used in treatment of different pathologies. OBJECTIVE: present paper evaluated the in vitro cytotoxic effect of this product in P3-X63/AG8/653 and Dunning R3327-G tumor lines from murine myeloma and rat prostate, respectively. METHODS: cytotoxic effect was evaluated by means of cellular growing kinetics and the metabolic damage. Doses of 1,10, 20, 50, 100, and 200 ìg/mL. RESULTS: poison had a cytostatic effect dependent of tumor line at issue. Doses effective varied among the cellular lines assessed. We studied also stability of product stored during 30 days at temperatures of -20° and 4° C, evidenced the loss of biological activity. CONCLUSIONS: we showed cytotoxic effect of crude poison of blue scorpion in cellular cultures.

Keywords : Rophalutus junceus; Escozul; cytotoxic effect; scorpions; cellular lines; in vitro..

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