Abstract

SUAREZ ROMAN, Gipsis; GONZALEZ GRIEGO, Antonio Mario; FERNANDEZ ROMERO, Tammy  and  ROMERO SAEZ, Mónica. Detection of IgG antibodies to ELISA-double-chain DNA using xenogenous, allogenic and autologous. Rev Cubana Invest Bioméd [online]. 2009, vol.28, n.1, pp. 0-0. ISSN 1561-3011.

Xenogenous DNA used as coating of an indirect enzyme-immunoassay for detection of IgG to double-chain DNA is very difficult to obtain and it is very expensive. OBJECTIVE: To assess usefulness of allogenic and autologous DNA coating antigen to determine these antibodies. METHODS: We used as coating the DNA from calf thymus, DNA from two subjects supposedly healthy, and DNA from 4 patients diagnosed with systemic lupus erythematosus (DLE), Analysis of antibodies presence was carried out in serum from 4 sick subjects. RESULTS: We achieved a 100% of coincidence in results, as well as a few differences in method discrimination ability using the three types of DNA. CONCLUSIONS: Human DNA (allogenic and autologous) is also useful as coating of this enzyme-immunoassay that the xenogenous DNA one.

Keywords : anti-DNAdch (double chain); ELISA (enzyme-linked immunosorbant assay); SLE (systemic lupus erythematosus)..

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