Abstract

SANCHEZ MIRANDA, Lilian; MORENO ZAMORA, Ana María; PEREZ BUENO, Tania  and  ESPINOSA CASTANO, Ivette. Optimization of a cultural medium for growing Gardnerella vaginalis. Rev Cubana Invest Bioméd [online]. 2009, vol.28, n.2, pp. 0-0. ISSN 1561-3011.

The optimization of the cultural medium Soy Triptone Broth supplemented with equine serum to grow Gardnerella vaginalis is presented in this paper. An experimental design 2² was used in which the influence of two components of this medium was evaluated; and a central design aimed at improving cell performances. With the best variant of this study, the runs allowed characterize the bacterial growth using different estimations. The greatest growths were observed in higher concentrations of soy triptone and equine serum. The variation of the evaluated component concentrations did not have an impact on the expression of antigenic proteins given by the obtained electrophoretic profiles.

Keywords : Gardnerella vaginalis; cultural medium; growth; optimization; experimental design.