Abstract

MARTINEZ, Olga M et al. Usefulness of lung-mouse animal model to evaluate the virulence of Shigella spp candidate vaccine straims. Vaccimonitor [online]. 2008, vol.17, n.1, pp. 27-30. ISSN 1025-0298.

ABSTRACT Shigella flexneri and Shigella sonnei, as other species of Shigella, are among the main etiological agents of acute diarrhoeal diseases worldwide, specially in developing countries, although we do not exclude developed ones, because of their low infective dose, between 10 and 100 colonies. This leads to the preparation of vaccines to prevent this illness and the need for an animal model to demonstrate the protective and immunogenic effectiveness of the shigellosis candidate vaccines. Many studies have been carried out since the intestinal enteropathy is difficult to demonstrate in monkeys and humans. The aforementioned and the capacity of Shigella sp to become resistant to antibiotic treatments motivated our study. In it we demonstrated the usefulness of the mouse-lung animal model to evaluate the virulence of vaccine candidates. Intranasal inoculation with Shigella flexneri and Shigella sonnei in concentration on between 107 and 109 CFU was used. The conclusion was that the lung-mouse animal model can result efficient for preclinical studies of any Shigella spp vaccine candidate.

Keywords : Shigella flexneri; Shigella sonnei; intestinal enteropathy; lung-mouse model.

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