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Vaccimonitor
On-line version ISSN 1025-0298
Abstract
ALVAREZ, Nadine et al. Purification of secretory immunoglobulin A from human colostrum. Vaccimonitor [online]. 2010, vol.19, n.3, pp. 26-29. ISSN 1025-0298.
Immunoglobulin A is the most abundant antibody isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory, and genitourinary tracts and in external secretions such as colostrums, breast milk, tears and saliva. In this study, secretory immunoglobulin A was obtained from human colostrum by a combination of chromatographic methods which was demonstrated by Dot blot. IgA fraction was analyzed by SDS-PAGE electrophoresis under reducing conditions, obtaining a high purity product by identifying the presence of secretory component, heavy and light chains of the immunoglobulin, with a migration pattern corresponding to their respective molecular mass.
Keywords : Human colostrum; Immunoglobulin A; secretory component.