Abstract

GARCIA, José et al. Strategies to obtain recombinant proteins in Escherichia coli . Vaccimonitor [online]. 2013, vol.22, n.2, pp. 30-39. ISSN 1025-028X.

Recombinant protein expression has been favored by the use of Escherichia coli due to its relatively low costs, high-density culture, easy genetic manipulation and also to compatible biotechnological tools. Strategies for recombinant expression in E. coli are presented in this paper. Genetic and physiologic factors are presented: number of copies of the expression vector, characteristic of the gene, ribonucleic acid (messenger) stability, promoter, host strain, clone stability, composition of the cultivation media, operation parameters in the bioreactor and cultivation and purification strategies.

Keywords : recombinant protein; Escherichia coli; genetic factors; physiologic factors; fermentation.

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