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Ingeniería Mecánica
versión On-line ISSN 1815-5944
Resumen
CARVAJAL-DE LA-OSA, Janet et al. Obtaining and characterizing collagen scaffolds from pigskin and chicken feet. Ingeniería Mecánica [online]. 2023, vol.26, n.1, pp. 1-10. Epub 12-Sep-2023. ISSN 1815-5944.
In the present work, three types of tubular scaffolds were obtained from pig skin collagen and chicken feet, which was isolated using a 0,5 molar acetic acid solution. The procedure used for the formation of the pores was lyophilization, which was carried out at a freezing temperature of -40 ºC. Tubular structure was achieved with steel molds, by modifying the mold dimensions to achieve wall thicknesses of 0,1; 0,5 and 1mm. Collagen was characterized by acrylamide gel electrophoresis tests and Fourier transform infrared spectrometry (FTIR). It was confirmed the presence of type I collagen in all cases. The collagen production process yielded 4,35 % starting with pig skin and 4.5% from chicken feet. The characterization of the scaffolds was carried out by determining the parameters of swelling, water absorption, biodegradability, as well as a morphological analysis by optical microscopy and finally the determination of the maximum resistance using a tensile test. As a result, chicken feet scaffolds had higher water absorption, lower biodegradability, lower swelling rate and lower strength than pig skin scaffold.
Palabras clave : scaffolds; collagen; lyophilization; protein electrophoresis; infrared spectroscopy; biodegradability; maximal strength.