Objective

: To evaluate the in vitro multiplication of Morus alba L., Criolla variety, in temporary immersion systems (SETIS™).

Materials and Methods

: Two trials were conducted for the evaluation of three immersion times (1,0; 3,0; 5,0 min.) and three immersion frequencies every four, six and eight hours. Afterwards, another experiment was conducted for the analysis of the morphological response of the sprouts, at 28, 45 and 60 days of cultivation. A complete randomized design was used, with three repetitions in all the experiments.

Results

: The results related to the immersion time showed significant differences in the evaluated variables for p < 0,05, except in the number of sprouts. In addition, it was found that the highest number of axillary buds was obtained in the immersion time of three minutes, with a value of 4,67. Meanwhile, in the length of the sprouts, the maximum value was achieved with three minutes of immersion (3,0 cm) with significant differences (p < 0,05) with regards to the other treatments. With the use of temporary immersion systems, with three minutes of immersion every eight hours, during 45 days of cultivation, the highest number of axillary buds and 1,90 sprouts were obtained; the sprout length reached 9,82 cm.

Conclusions

: In the sprouts no morphophysiological changes were observed, ascribed to the cultivation conditions. It was proven that the in vitro micropropagation of mulberry, Criolla variety, in temporary immersion systems SETIS™, is possible.

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