Abstract

SIJO YERO, Mariela G; VALDES DIEZ, Lilliam; GONZALEZ GARCIA, Ángela  and  FARIAS DOMINGUEZ, Yaimí. Optimización del juego de reactivos para la determinación de urea en suero: Método Berthelot modificado. Rev Cubana Farm [online]. 1997, vol.31, n.3, pp. 155-158. ISSN 1561-2988.

The process of optimization was developed in a technique of analysis and in one formulation based on the Berthelot method modified with sodium salicilate, which was designed for the determination of such analyte in serum. The objectives evaluated were the following: color reactive solution, alkaline reactive solution, reaction temperature, color development time, hydrolisis time, enzimatic activity of urease, and wave lenght. The optimal values of each of these parameters were established. Lineality was attained in a range of urea concentrations from 3.33 to 26.6 mmo/L. A variation coefficient of 4.8 % was obtained in an accuracy study

Keywords : REAGENT KITS, DIAGNOSTIC [standards]; UREA [blood]; EFFECTIVENESS.