Abstract

TORANO PERAZA, Gilda et al. Evaluación de reactivos cubanos elaborados para el diagnóstico rápido de Haemophilus influenzae tipo B. Rev Cubana Farm [online]. 2001, vol.35, n.2, pp. 109-115. ISSN 1561-2988.

A latex reagent and an antiserum for counterimmunoelectrophoresis (CIE)and capsular swelling (CS), both produced by the Acute Bacterial Respiratory Infections Lab of "Pedro Kourí" Institute were tested in the detection of a Haemophilus influenzae type B- specific capsular antigen. The reagents were evaluated in the serotyping of 786 Hib strains isolated from invasive diseases and nasopharyngeal carriers and in the rapid diagnosis of cerebrospinal fluid, serum and urine samples taken from 258 patients with clinical diagnosis of meningoencephalitis. Satisfactory sensitiviy and specificity indexes were obtained, which were determined by using Hib antiserum traded by DIFCO for CS technique and CIE and latex reagent Wellcogen for the Hib diagnosis as a reference. This paper proposes the validation of both reagents by authorized national bodies so that they can be introduced in the Cuban health care network.

Keywords : HAEMOPHILUS INFLUENZAE TYPE B [isolation & purification]; INDICATORS AND REAGENTS; MENINGOENCEPHALITIS [diagnosis]; LATEX FIXATION TESTS; COUNTER INMUNOELEC-TROPHORESIS; HAEMOPHILUS INFECTIONS [diagnosis].

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