Abstract

DIAZ MACHADO, Leida; GIRALDINO, Isabel  and  DAVALOS PANTOJA, Lourdes. Reacción de inmunoglobulinas de distintas especies con el factor reumatoideo en inmunoensayos de látex aglutinación. Rev Cubana Farm [online]. 2003, vol.37, n.3, pp. 1-1. ISSN 1561-2988.

The rheumatoid factor is considered as the fundamental responsible for the interferences (false +) produced in the immunoassays, since it reacts unspecifically with different epitopes of the molecules of immunoglobulin G (IgG). This paper was aimed at studying the IgG from 7 species (human, rabbit, donkey, lamb, horse, hen and mouse; and of the latter the subclasses IgG1, IgG2a, IgG2b). Particles of polystyrene of 0.8 m that were covered with 3 concentrations (0.02, 0.01. 0.05 mg/m2) of every 1 g were used. Reactivity was evaluated against 6 concentrations of rheumatoid factor (1 000, 500, 250, 64, 16, 8 UI). In all cases, it was demonstrated that the reactivity decreases as the covering concentration diminishes and that the decreasing reactivity order was: human, rabbit, donkey, lamb, and horse; whereas for the murine IgG it was IgG1, IgG2a and IgG2b. The hen IgG did not react against the rheumatoid factor. It was the ideal species to make latex reagents to avoid the interferences of the rheumatoid factor.

Keywords : RHEUMATOID FACTOR; IMMUNOGLOBULINS; IMMUNOASSAY; MICROSPHERES.