Abstract

GARCIA BORGES, Lisandra; PEREZ PRIETO, Teresa María  and  VALDES DIEZ, Lilliam. Validation of ultraviolet method to determine serum phosphorus level. Rev Cubana Farm [online]. 2009, vol.43, n.4, pp. 45-52. ISSN 1561-2988.

Validation of a spectrophotometry method applicable in clinic labs was proposed to analytical assessment of serum phosphates using a kit UV-phosphorus of domestic production from "Carlos J. Finlay" Biologics Production Enterprise (Havana, Cuba). Analysis method was based on phosphorus reaction to ammonium molybdenum to acid pH to creating a measurable complex to 340 nm. Specificity and precision were measured considering the method strength, linearity, accuracy and sensitivity. Analytical method was linear up to 4,8 mmol/L, precise (CV < 3 %) and exact (% recovery 98 to 102 %; r ³ 0.999) during clinical interest concentration interval where there were not interferences by matrix. Detection limit values were of 0.037 mmol/L and of quantification of 0.13 mmol/L both were satisfactory for product use.

Keywords : Phosphorus; spectrophotometry; clinical lab; clinical diagnosis.

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