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Revista Cubana de Farmacia

Print version ISSN 0034-7515On-line version ISSN 1561-2988


ARENCIBIA ARREBOLA, Daniel Francisco; ROSARIO FERNANDEZ, Luis Alfredo  and  HERNANDEZ RODRIGUEZ, Yanet. Comparison between induced and spontaneous chromosomal aberrations in OF-1 and C57BL/6/cenp bone marrow mice. Rev Cubana Farm [online]. 2010, vol.44, n.4, pp.503-511. ISSN 0034-7515.

The in vivo cytogenetics array is a very sensible short term mutagenesis method useful to detect mainly the in vivo the structural chromosomal aberrations. The aim of present paper was to determine the more effective experimental bio-model in this type of model to compare the spontaneous and induced frequency of the above mentioned aberrations in bone marrow cells in both sexes of two mice species (OF-1 and C-57BL/6/cenp). Four experimental groups were designed, one of negative control, twotreated during 14 days using oral vehicle substancesm NaCI (0.9 %) and tween 65 (2 %) and one of positive control treated at 48 and 24 h before sacrifice using cyclophosphamide (50 mg/kg) by intraperitoneal route. The OF-1 mice species had the lowest spontaneous frequency in variables analyzed that the C-57BL6/cenp species, achieving a lower spontaneous number of total cells with aberrations and a great sensitivity to cyclophosphamide than the C-57BL/6/cenp species. We conclude that the OF-1 and C-57BL/6/cenp mices were good models to be used in genotoxicological studies due to the low spontaneous frequency of the above mentioned aberrations and the numerical ones analyzed, thus it is recommended the use of OF-1 species.

Keywords : Spontaneous frequency; induced frequency; chromosomal aberrations; OF-1 mice, C-57BL/6/cenp mice; Cyclophosphamide; bone marrow.

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