Abstract

ARBOLEDA ECHAVARRIA, Carolina  and  MEJIA GALLON, Amanda Inés. Induction of lacasa activity in Ganoderma sp and the antioxidant activity of its biomass. Rev Cubana Farm [online]. 2010, vol.44, n.4, pp. 519-532. ISSN 1561-2988.

The Basidiomycetes have a great potential for the production of wide application enzymes in the pharmaceutical, alimentary, food industry and the water and soils recovery. In Colombia come types of Basidiomycetes fungi including the Ganoderma.sp that is additionally worldwide recognized by its medicinal effects. In present paper authors assessed the influence of invironment pH and of the inducers: copper and Angustifolia, kunth sawdust in the production of lacase enzyme and also in the antioxidant activity of DPPH and ABTS^.+ radicals. The variables analyzed at three levels by Design Expert 6 software demonstrated that the better combination of variables for a maximal enzyme expression was obtained using the 0.75 % Angustifolia kunth sawdust, 250 mM of copper and pH 5; the great percentage of the ABTS radical inhibition (86 %) was observed at a pH 4 without copper addition and with a percentage of sawdust of 1.50 and the greater percentae of DPPH radical inhibition (57 %) was observed at a pH 6 without sawdust and copper addition.

Keywords : Ganoderma; lacase; antioxidant activity.

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