Abstract

DURAN LENGUA, Marlene; GAITAN IBARRA, Ricardo  and  CANO DURAN, Larissa. Effects of extracts from Neopetrosia rosariensis on myeloperoxidase and on elastase by using the neutrophil model. Rev Cubana Farm [online]. 2014, vol.48, n.1, pp.146-155. ISSN 0034-7515.

Introduction: neutrophils are the first line of defense, releasing their granular contents made up of reactive oxygen species-generating enzymes such as myeloperoxidase and proteolytic enzymes such as elastase, which degrade elastin and collagen. Objective: to evaluate toxicity of marine sponge Neopetrosia rosariensis extracts and their action as inhibitors of neutrophil degranulation and inhibition of the catalytic activity of human neutrophil elastase. Method: the cytotoxicity was assessed by Trypan blue exclusion test of cell viability. Degranulation inhibition was evaluated by reduction of myeloperoxidase release percentage and inhibition of catalytic activity of elastase was determined by reduction of enzyme activity on specific substrate. Results: the extracts exhibited low toxicity at the evaluated concentrations, with cellular viability percentage exceeding 80 % even at the highest concentration (25 µg/mL). They also showed inhibitory action of myeloperoxidase release, the greatest effect being concentrated on the most polar extracts (total and partial methanolic). The inhibition of the elastase activity was mostly found in the lowest polarity extracts (hexane and partial dichloromethane) at concentrations of 10 and 25 µg/mL, according to the hydrophobic character of the active site of the enzyme. Conclusions: these results of the evaluated extracts are encouraging, considering each extract represents a complex mixture of active compounds of 10-25 µg/mL. This arouses great interest to continue their fractioning as potential agents for possible therapy of inflammatory processes.

Keywords : antinflammaroty activity; Neopetrosia rosariensis; polymorphonuclear myeloperoxidase and elastase.

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