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Revista Cubana de Pediatría

Print version ISSN 0034-7531On-line version ISSN 1561-3119

Abstract

PARADOA PEREZ, Martha Leonor; MONTOTO LIMA, Armando Néstor  and  MARRERO RODRIGUEZ, Rosa María. Cuantificación de inmunoglobulinas mediante el espectrofotómetro Shimadzu 160-A. Rev Cubana Pediatr [online]. 1997, vol.69, n.2, pp.124-128. ISSN 0034-7531.

The fixed time turbidimetric procedure for Hitachi system was adapted to the spectrophotometer Shimadzu 160-A. The quality control was carried out with products of national and international reference to check precision and accuracy. The results of the means (IgG=12.31, IgA=2.13 and IgM = 1.38) and standard deviations (IgG=0.3885, IgA=0.155 and IgM=0.129) show a little dispersion and ensure the validity of our method. With this study, it is possible to put into practice this technique by a fast and efficient method in an equipment that is widely used in Cuba and much cheaper than the Hitachi.

Keywords : IGG [blood]; IGA [blood]; IGM [blood]; QUALITY CONTROL; NEPHELOMETRY AND TURBIDIMETRY [instrumentation].

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