Abstract

MONTES DE OCA, Nivian et al. CULTURE COLLECTION DEVELOPMENT AND ESTABLISHMENT AT CENSA. Rev Salud Anim. [online]. 2008, vol.30, n.1, pp. 17-24. ISSN 0253-570X.

The use of the microorganisms has been a key when facing and solving serious problems in humanity. This study involves the use of living cultures, and if their importance is proved, they should be maintained and preserved in a collection. For the establishment and operation of such collection at the National Center for Animal and Plant Health (CENSA), the requirements of the World Federation Culture Collection (WFCC, 1999) ISO 9001:2000, the Regulations of the Good Manufacture and Laboratory Practices, as well as the government dispositions for the management of biological agents were used. Such establishment allowed the development of aspects like: documentation, identification, authenticity, purity, viability and stability of strains; maintenance of equipment; procedures supplying and exchanging strains; long term security; control of the environmental conditions; audits; fulfilment of the legislations; staff; training and biosafety. Practical recommendations have been offered for the people in charge of collections of microorganisms and for the establishment of a Quality System that assures the management of biological agents which participate in the research, production and service processes offered by the scientific institution of the country.

Keywords : Microbial Culture Collection; microorganism; preservation.

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