Revista de Salud Animal
version ISSN 0253-570X
RODRIGUEZ, Edisleidy et al. ISOLATION OF SWINE TRANSMISSIBLE GASTROENTERITIS VIRUS IN CELL CULTURES. Rev Salud Anim. [online]. 2008, vol.30, n.2, pp. 93-97. ISSN 0253-570X.
Transmissible gastroenteritis (TGE) is an infectious, acute and highly contagious disease in swine. It is caused by a virus belonging to the Coronaviridae family, Coronavirus genus. Some outbreaks starting with diarrheic symptoms with the clinical characteristics of TGE disease have been present in our country since 2003. But for having the confirmation of the diagnostic, it was necessary to isolate the virus in cell cultures from sick animals, followed by an identification in those cell cultures with cytopatic effect. That is why, it was necessary to develop a methodology for the virus isolation, since for this virus, it has been previously reported that isolation in cells is very difficult to obtain. In this research, a methodology was developed for the virus isolation. The isolates obtained during the technique evaluation were identified as positive by indirect immunoperoxidase in cells with an anti TGE specific commercial monoclonal antibody
Keywords : transmissible gastroenteritis virus; TGE; virus isolation.