Revista de Salud Animal
version ISSN 0253-570X
ESCOBAR, A; ABELEDO, María Antonia; VEGA, E and CEDENO, V. DETERMINATION OF AIVLOSIN IN FEEDSTUFFS BY HIGH PERFOMANCE LIQUID CHROMATOGRAPHY (HPLC). Rev Salud Anim. [online]. 2008, vol.30, n.2, pp. 116-121. ISSN 0253-570X.
A procedure was developed by high perfomance liquid chromatography (HPLC) in order to determine Aivlosin in feedstuffs with destination to different productive categories of pigs. Aivlosin was extracted with acetonitrile and concentrated five times when the concentration was inferior to 20 ppm . The extract was analyzed by HPLC using a reverse phase column C18 with UV detection (280 nm) and acetonitrile:amoniun acetate 0.15M:acetic acid (45:45:10v/v/v) as eluent. The validation results showed a linearity between 5-80 µg/ml with a general equation of Y= 2.60 +1.72X, where the regression coefficient was 0.999 (p < 0.05) with a variation of the confidence interval of the slope between 1.57-1.87. Limits of detection and quantification were 4 and 12 µg/g respectively when the sample was concentrated five times. While the sample applied directly presented a limit of detection of 42 µg/g. Average recovery of Aivlosin was 63±7.3 and 99±6.5% when the sample was directly applied and concentrated. The precision of the method measured through the variation coefficient in both procedures was inferior to 15%. All the samples analyzed fulfilled the quality specification offered by the producer.
Keywords : Aivlosin; HPLC; feedstuffs.