Revista de Salud Animal
version ISSN 0253-570X
ESCOBAR, A et al. DETERMINATION OF CHLORTETRACYCLINE IN FEEDSTUFFS BY HIGH PERFOMANCE LIQUID HROMATOGRAPHY (HPLC). Rev Salud Anim. [online]. 2008, vol.30, n.2, pp. 122-127. ISSN 0253-570X.
A procedure was developed by High Perfomance Liquid Chromatography (HPLC) in order to determine chlortetracycline in feedtuff destined to different productive categories of pigs´ breeding. Chlortetracycline was extracted with acid acetone solution. The extract was analyzed by HPLC using a reverse phase column C18 with UV detection (340 nm) and dimethylformamide to 27% as eluent. The validation results showed a linearity between 50-1000 µg/mL with a general equation of Y= 0.23X _2.62, where the regression coefficient was 0.996 (p < 0.05) with a variation of the interval of trust of the slope between 0.23-0.24. Limits of detection and quantification were 33.and 50 µg/g respectively. Average recovery of chlortetracycline was 90.2±7.0%.The precision of the method in both procedures was inferior to 15%. All the samples analyzed between 300-450 µg/g fulfilled the quality specification offered by the producer, while the samples with 150 ug/g did not complete their specification showing values among 66-123 ug/g.
Keywords : chlortetracycline; HPLC; feedstuff.