Abstract

BETANCOURT, A; RODRIGUEZ, Edisleidy; RELOVA, Damarys  and  BARRERA, Maritza. BIOLOGICAL CLONING OF A BOVINE CORONAVIRUS ISOLATE. Rev Salud Anim. [online]. 2008, vol.30, n.3, pp. 160-165. ISSN 0253-570X.

In order to obtain a biologically cloned bovine coronavirus isolate, the isolate VB73/04 was adapted to multiplication in MDBK cell line. This isolate induced the formation of plaques, which were homogeneous after biological cloning. The viral population obtained was tested for bovine coronavirus by RT-PCR assay and seroneutralization.

Keywords : bovine coronavirus; biological cloning; winter dysentery.