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Revista de Salud Animal

Print version ISSN 0253-570X


BOLIVAR, Ana María et al. Detection of hemotropic agents in a livestock farm using PCR and DGGE. Rev Salud Anim. [online]. 2014, vol.36, n.1, pp.53-57. ISSN 0253-570X.

This paper reports the results of a diagnostic evaluations by PCR for hemotropic agents in buffalo and cattle (n=77), existing in the same farm. There was a positivity of 74.03% of hemoparasite infection for the total population studied, prevailing Anaplasma marginale on Trypanosoma theileri, Trypanosoma vivax and Babesia bigemina, respectively. Additionally, some DNA problems were tested by DGGE, revealing the presence of A. marginale and Mycoplasma wenyonii. The results obtained confirm a presumptive clinical situation and the validity of the molecular method.

Keywords : cattle; buffalo; hemotropic agents; PCR; DGGE.

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