Abstract

HERNANDEZ-FILLOR, Rosa Elena; BAEZ-ARIAS, Michel; ALFONSO-ZAMORA, Pastor  and  ESPINOSA-CASTANO, Ivette. Antimicrobial susceptibility and biofilm formation in Escherichia coli isolates from laying hens. Rev Salud Anim. [online]. 2017, vol.39, n.3, pp. 00-00. ISSN 2224-4700.

Escherichia coli is an important pathogen in poultry associated with enteric and respiratory processes, causing significant losses due to the decrease in the laying and costs in medicines. Plasmids and chemosomes encoding resistance mechanisms in E. coli commensal or pathogenic strains can be transferred to clinically important bacteria in human health through the food chain. In addition to the acquired resistance, the formation of biofilm gives the strains tolerance to antibiotics, which contributes to the recurrence of infections. The objective of this work is to determine the susceptibility to antibiotics of E. coli strains from laying hens. One hundred and eighty three sewage exudates were collected from four productive units in Mayabeque province. Eighty three isolates such as E. coli were identified. The susceptibility in vitro to ten antibiotics was determined by the Agar Diffusion Method and the ability to form biofilm by the Plate Adhesion Method. The Minimum Inhibitory Concentration for the planktonic growth (MIC_P) and for the inhibition of biofilm formation (MIC_B) was determined. The highest percentages of resistance by the disc diffusion test were to Oxytetracycline, Norfloxacin, Ticarcillin/Clavulanic Acid, Cefoxitin and Ceftriazone; and the lowest percentages to Gentamicin, Colistin and Nitrofurantoin. Of the total of E. coli isolates, only 14 % formed biofilm in vitro. MIC_B was higher than MIC_P, which shows that biofilm formation contributes to tolerance to antibiotics

Keywords : biofilm; Escherichia coli; laying hens; antibiotic resistance.

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