Abstract

DIAZ-SANCHEZ, Adrian Alberto; MARTINEZ-MARRERO, Siomara  and  CORONA-GONZALEZ, Belkis. Multi-locus sequence typing applied to the molecular characterization of hemoparasites in cattle. Rev Salud Anim. [online]. 2018, vol.40, n.1, pp.00-00. ISSN 0253-570X.

Molecular markers are valuable tools for researches on the genetic diversity, population and evolutionary structure of important infectious agents, and they have a great impact on the design and implementation of control strategies. For the typing of pathogenic microorganisms, several methods have been developed differing in the discriminative power, reproducibility and easy of interpretation. The multi-locus sequence typing was proposed in 1998 as a universal and definitive method for the characterization of bacteria, using the pathogen Neisseria meningitidis as the object of study. There is currently an increasing number of multi-locus sequence typing protocols developed and used in epidemiological researches at different scales, as well as in biology and structure studies of different microbial populations, pathogenicity analysis and bacterial evolution. In the present work, the main typing schemes based on this methodology for the study of hemoparasites that affect cattle

Keywords : molecular markers; multi-locus sequence typing; PCR; hemoparasites; cattle.

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