Abstract

MARTINEZ-BARBABOSA, Ignacio et al. Presence of Blastocystis spp. in the mollusc Crassostrea virginica, in Mexico City. Rev Salud Anim. [online]. 2018, vol.40, n.2 ISSN 2224-4700.

The aim of this study was to identify, by microscopic analysis and polymerase chain reaction (PCR), the presence of Blastocystis spp. in the mollusc Crassostrea virginica sold in Mexico City. Thirty specimens of the mollusc were obtained. The intestine of each was dissected and Blastocystis spp. was isolated from the faeces for microscopic analysis. Of those samples positive to Blastocystis, 15 (50 %) were selected, the genomic DNA was extracted and the PCR amplification of the 26S rRNA gene was performed. Of all the samples, a fragment of 1 770 bp was amplified in 13 of them. These results show the presence of Blastocystis spp. in the oysters.

Keywords : Blastocystis spp.; Crassostrea virginica; oysters; PCR.

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