Abstract

RODRIGUEZ, Yakelín; FERNANDEZ, Kalyanne; MENA, Aracelys  and  MARENTES, Francys L. Respuesta de enzimas antioxidantes y crecimiento de vitroplantas de papa micorrizadas In Vitro. cultrop [online]. 2008, vol.29, n.1, pp. 29-35. ISSN 0258-5936.

The technology used for potato (Solanum tuberosum) micropropagation still has some problems, so that it does not fully guarantee the conditions for the further development of derived plants under natural environments. Inoculation with arbuscular mycorrhizal fungi (AMF) at in vitro phase could be an alternative to eliminate some of these problems, by its positive influence on vitroplants obtained with a greater ability of survival and adaptation. This work was aimed at evaluating the behaviour of antioxidant enzymes -peroxidase, polyphenoloxidase and superoxide dismutase- and vitroplant growth mycorrhized in vitro with two AMF species: Glomus mosseae and Glomus hoi-like. Thus, potato vitroplants of Desirée cv. were cultivated in sterile containers with a mixture of vermiculite and twice the components of M (minimum) culture media. In this stage, disinfected spores of both AMF were inoculated, which together with the control without inoculation constituted the three treatments studied. Once mycorrhized, vitroplants were transferred to the acclimatization phase and transplanted to pots. Results indicate G. mosseae is the best species in both phases; since greater intraradical fungal growth as well as superior values of aerial fresh mass and leaf number were observed in plants inoculated with this species. Differences detected among treatments were biochemically corroborated with the changes found in enzymatic activities and mainly in isoenzymatic expression. This suggests the use of AMF for obtaining micropropagated potato plants mycorrhized at in vitro stage, which should have a greater ability for survival and adaptation

Keywords : Solanum tuberosum; fungi; arbuscular mycorrhizae; micropropagation; enzymes; antioxidants.

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