Abstract

CASTILLO, J. G et al. Determinación de la estabilidad genética en cuatro especies del banco de germoplasma de papa en Cuba conservadas In Vitro. cultrop [online]. 2010, vol.31, n.3, pp.00-00. ISSN 0258-5936.

Four in vitro-preserved potato species were studied for ten years, with the aim of determining if they kept their genetic stability when compared to those from the base collection remaining in the field. Species of both origins were morphologically described; isozymatic and molecular methods were used to evaluate if changes took place in the band patterns of in vitro-preserved species. The isozymatic systems, peroxidases, esterases, polyphenol oxidases and carbon anhydrase, and the AFLP molecular marker were employed. The four preserved species used were: Solanum schruteri Buk, Solanum dolichostigma Buk, Solanum jamesii Jorr and Solanum tuberosum L. Seedlings were preserved in a slow growth medium and sub-cultivated twice a year. The qualitative characters, yield and its components were evaluated, to determine if there were phenotypical changes among the species studied. Five primer combinations were used in the AFLP analysis. A t test of media comparison was made to yield and its components, to calculate if there were differences among accessions. There were not any remarkable phenotypical changes for the qualitative characters, yield and its components. Isozymatic systems did not detect changes in the band patterns, while AFLP analysis showed notable differences concerning band number; thus, it is concluded that genetic stability is not maintained in the different species studied during the preservation period evaluated.

Keywords : potato; germplasm; genetic stability; molecular markers.

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