Abstract

PEREZ-ALVAREZ, Sandra et al. Contaminant fungi in in vitro establishment of potato apexes. cultrop [online]. 2016, vol.37, n.4, pp.84-88. ISSN 0258-5936.  http://dx.doi.org/10.13140/RG.2.2.26063.69284.

Potato (Solanum tuberosum L.) is one of the most important food sources worldwide and it is affect for some pathogen either in the field or in the storage conditions. Some of the pathogens that attack this crop may remain in the tuber and they can be taken to the field at the time of planting so it is very important to obtain high quality potato seed for which in vitro culture is a useful technology. The aim of this work was the identification of fungal contaminants at the in vitro establishment phase of apexes of two potato variety (Fianna and Atlantic). Minitubers were storage in the dark at 18 ^oC for 20 days to obtain apexes that were collected, disinfected and placed in Murashige and Skoog medium supplemented with sacarose and agar. After seven days of apexes establishment, 100 % of the samples showed contamination and damage inside, which were identified in an optical microscopy as Fusarium and Penicillium genera, both tuber pathogen. This research demonstrate once again the importance to have healthy and high quality material for in vitro culture and the absence of sanity in the material sold to the farmers to obtain pre-basic seed or G1

Keywords : culture; Fusarium; Penicillium.

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