Abstract

VILLALOBOS OLIVERA, Ariel et al. Cryogenic strategy for the establishment of a germplasm bank of pineapple (Ananas comosus var. Comosus). cultrop [online]. 2016, vol.37, suppl.1, pp. 81-90. ISSN 1819-4087.

In pineapple have been used cryopreservation protocols based on a vitrification procedure. However, the influence of some technical factors is still unknown to achieve a strategy cryogenic routine application to a wide range of genotypes. Furthermore, there has been no histological analysis to display any structural changes during a process of cryopreservation. In the present investigation different technical aspects of a cryogenic strategy are determined to increase survival levels from the apexes to immersion in liquid nitrogen. These were: type of apex, consisting of 3-4 leaf primordia with an approximate size of 2,5-3mm; preculture 0,3 mol L^-1 sucrose; application of the loading solution 0,4 mol L^-1+2 0,4 mol L^-1 mol glycerol; application temperature of the vitrification solution PVS2 at 0 °C for 420 min. The analysis of the display of structural changes in the cryopreserved apices revealed that the meristematic cells in the apical dome and leaf primordia in formation, suffered few cellular alterations and kept almost intact its morpho-physiological characteristics in the best conditions of survival. The method of vitrification was applied successfully for 9 accessions of the in vitro genebank of Bioplant center. This is an important step for the establishment of a gene bank for long-term cultivation of pineapple

Keywords : apices; germplasm; histology; vitrification.

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