Abstract

MARTINEZ-ESPINOSA, Yunieski; PEREZ-RODRIGUEZ, Juan L; SANTIESTEBAN-TOCA, Cosme E  and  BAEZ-PEREZ, Evelio L. Computer system to count seeds of Nicotiana tabacum. cultrop [online]. 2016, vol.37, suppl.1, pp.106-110. ISSN 0258-5936.

In Cuba, conservation of Nicotiana tabacum is vital because it is one of the main items on the export and sustainability of the economy. The experimental station of Cabaiguán in Sancti Spiritus, whose functions include the conservation of genetic material from Nicotiana tabacum. Due to the small size of these seeds (0,1-0,5 mm), quantification has become one of the main problems, showing inconsistency in results of the count (high error rate) of a specialist to another, labor intensive and time lost. Therefore, it is propose to develop a computer system for quantification of Nicotiana tabacum seeds, based on techniques of digital image processing. Seedsimagesare captured ingray levelsina conventionalscanner. Image segmentation is performed by thresholding the histogram from the frequency of gray levels. Impurities and image noise are eliminated from size. The average seed size and number of seeds present in each cluster is calculated. Finally, the number of seeds in the image is determined. To assess the quality and effectiveness of the proposed system an experimental validation was performed on 15 images containing 1492 seeds for different resolutions (150 dpi, 300 dpi, 600 dpi and 1200 dpi). Statistical results prove the ability of the system to count seeds with an accuracy above 99 % for the same or higher than 300 dpi resolutions

Keywords : quality; genetic material; digital image processing.

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