Abstract

CALANA-JANEIRO, Víctor M. et al. Disinfection of pepper seeds (Capsicum annuum L.) cultivar ‘YAMIL’ for in vitro implantation. cultrop [online]. 2019, vol.40, n.3  Epub Sep 01, 2019. ISSN 1819-4087.

In the Department of Genetics and Plant Improvement of the National Institute of Agricultural Sciences, an experiment was carried out with pepper seeds (Capsicum annuum L.) cultivating ‘YAMIL’, in which different concentrations of sodium hypochlorite were compared ( NaOCl) [1.25; 2.5 and 5 %] and disinfection times (one, three and five minutes) of the pepper seeds cultivate ‘YAMIL’ for later implantation in vitro, in two culture media, which contained the salts of the basal culture medium of Murashige and Skoog (MS), supplemented with sucrose (15 and 30 g L-1), in order to determine the best treatment and disinfection time of the seeds of this pepper cultivar. The results obtained in this work indicated that 100% of the seeds were disinfected with 2.5 % NaOCl for three minutes or 5 % for three minutes. 100 % of the seeds germinated and the highest seedling height (5.84 cm), as well as the number of roots per seedling (5.15) and their vigor, showed the best results with the use of NaOCl (5 %) for one minute and the use of the culture medium MS supplemented with sucrose (30 g L-1) that were statistically superior to the control treatments. From the results obtained, a disinfection methodology is obtained for the in vitro implantation of the ‘YAMIL’ cultivar pepper.

Keywords : contamination; germination; vegetable; sodium hypochlorite; sucrose.

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