Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
ESPINO, ANA MARGARITA; PADRON, LORENZA; DUMENIGO, BLANCA and LAFERTE, JOSÉ. UltramicroELISA indirecto para la detección de anticuerpos IgG en pacientes con fascioliasis. Rev Cubana Med Trop [online]. 1997, vol.49, n.3, pp. 167-173. ISSN 1561-3054.
The standardization of an ultramicroELISA for the detection of IgG antibodies anti excretory-secretory antigens of Fasciola hepatica (UME-Fasciola) is described. It was studied a considerable group of sera of which 56 were from patients with fascioliasis, 168 from patients with other parasitic diseases, and 300 from sound persons that were used as negative controls. As regards the parasitology testconsideredas"GoldS tandard",theUME-Fasciola showed a sensitivity of 100 %, an specificity of 98 %, and predictive values for positives and negatives of 90.3 % and 100 %, respectively. Cross-reaction was only observed with the sera from patients infected with Opistorchis felineus. On comparing the UME-Fasciola with the conventional ELISA, it was obtained a concordance index of 95.5 %.
Keywords : IGG [analysis]; ANTIBODIES, ANTI-IDIOTYPIC [analysis]; FASCIOLA HEPATICA [immunology]; ANTIGENS, HELMINTH [diagnostic use]; ANTIGENS, HELMINTH [immunology]; FASCIOLIASIS [immunology]; FASCIOLIASIS [diagnosis]; ENZYME-LINKED IMMUNOSORBENT ASSAY [standards]; ENZYME-LINKED IMMUNOSORBENT ASSAY [methods].