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Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
Abstract
RODRIGUEZ LAY, Licel de los Ángeles et al. Toma de muestra en papel de filtro para la detección de anticuerpos IgM antivirus de la hepatitis A. Rev Cubana Med Trop [online]. 1998, vol.50, n.1, pp. 42-47. ISSN 1561-3054.
The usefulness of the whole sample taking in filter paper for the detection of anti-HAV IgM is described. 168 serum and filter paper samples were simultaneously compared. No alterations were observed in the specificity of the enzyme-linked immunosorbent assay for the catchment of IgM in the 52 samples of the control group or in the other 116 from patients with clinical suspicion of acute viral hepatitis. A sensitivity, specificity and coincidence of 100 % was obtained on comparing the filter paper with the dilution 1:500 with the serum. The correlation established between the values of optical density of the positive results to anti-HAV IgM, the filter paper at dilution 1:500 and the serum was of 0.895. On applying this type of sample taking in a group of hospitalized patients, 100 % of coindicence with clinical suspicion was attained. Besides, it was proved its usefulnes for the diagnosis of a viral hepatitis outbreak. According to our results, it is recommended the introduction of this type of sample taking in the diagnosis of hepatitis A.
Keywords : ENZYME-LINKED IMMUNOSORBENT ASSAY [methods]; VIRAL HEPATITIS, HUMAN [blood]; VIRAL HEPATITIS, HUMAN [epidemiology]; DISEASE OUTBREAKS; IGM [analysis].