Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
AVALOS REDON, Ivonne et al. Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa. Rev Cubana Med Trop [online]. 1998, vol.50, n.2, pp. 100-104. ISSN 1561-3054.
The polimerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1 - 97 pb, Sabin 2 - 71 pb, Sabin 3 - 44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. Tghis study provided one more evidence about the non circulation of the wild poliovirus in Cuba.
Keywords : POLIOVIRUSES [isolation & purification]; POLIOVIRUSES [genetics]; POLYMERASE CHAIN REACTION [methods]; RNA, VIRAL [isolation & purification].