Abstract

FERRIOL MARCHENA, Xenia et al. Validación de un ELISA para la cuantificación de IgG humana antiproteína de Neisseria meningitidis serogrupo B. Rev Cubana Med Trop [online]. 1999, vol.51, n.2, pp. 99-105. ISSN 1561-3054.

An indirect ELISA test was optimised in which B meningoccocus outer membrane proteins included in the Cuban Vaccine known as VA-MENGOC-BC were used as capture antigents. Specific antibodies in serum samples from vaccinated individuals were detected using a human anti-IgG conjugate, i,e, alkalyne phosphatase, thus the reaction developed with a specific substrate called p-nitrophenilphophate. Standard serum was obtained from a reference standard, the gage curve range was 625 to 20 000/mL. The control serum was selected from the most interesting areas for the samples, hence, assay characteristics were determined. Intra-assay, inter-assay and total inaccuracies were lower than 10 % in the most linear area of the curve. Detectability was 700/mL. Recovery, paralelism and linearity studies showed an under 10 % innaccuracy.

Keywords : NEISSERIA MENINGITIDIS; MENINGITIS MENINGOCOCCAL [diagnosis]; MENINGITIS MENINGOCOCCAL [inmunol]; MENINGITIS MENINGOCOCCAL [therapy]; ENZYM-LINKED; IMMUNO-SORBET ASSAY [methods]; VACCINES.

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